ExFLP-19 Determination of Extraneous Material in White Wheat Flour
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A546FA5A9F5F4CE0B5836572B7B67380 |
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0.03 |
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页数： |
7 |
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日期： |
2012-3-3 |
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Published by: POLYSCIENCE PUBLICATIONS, P.O. Box 1606, Station St-Martin, Laval, Quebec,Canada H7V 3P9. TEL.: 1-800-840-5870. FAX: (450) 688-1930.,Government of Canada Gouvernement du Canada,Laboratory Procedure ExFLP-19,April 1997,HEALTH PROTECTION BRANCH,OTTAWA,DETERMINATION OF EXTRANEOUS MATERIAL IN WHITE WHEAT FLOUR,M.-A. Rivers,Research Division, Bureau of Microbial Hazards,Food Directorate, Postal Locator: 2204A2,Health Canada, Ottawa, Ontario, K1A 0L2,1. APPLICATION,This method is applicable to the sampling and examination of white wheat flour for insects, rodent hairs and,other extraneous material to determine compliance with Sections 4, 5 and 7 of the Food and Drugs Act. This,method replaces ExFLP-19 dated September 1987.,2. DEFINITION OF TERMS,A lot is defined as that amount (volume, weight, etc.) of the food which is produced, stored and/or shipped,under conditions as nearly uniform as possible, preferably designated by a common container code or,marking, and, in any event, consisting of not more than one variety, grade or type of product from a single,identifiable source.,3. COLLECTION OF SAMPLES,3.1 Scrutinize the entire lot for live infestation. If live infestation is found, do not sample until after,fumigation or other effective treatment.,3.2 Obtain three sample units selected at random from the lot of at least 50 g each using appropriate,sampling equipment and containers. Three sample units constitute a sample.,3.3 Each sample unit must be kept separate and labelled 1, 2 and 3. Complete information respecting,the lot size, weight of individual containers, country of origin, exporter, importer, or domestic,manufacturer, and product and lot identification should be recorded and should accompany the,sample.,4. MATERIALS AND SPECIAL EQUIPMENT,1) Balance,2) 2-2.0 L beakers and lids (watch glasses),3) Autoclave. Either (a) slow exhaust type (Set slow exhaust to lower pressure from 1.15 to 0 kg per sq.,cm. in 15-20 min) or (b) non-slow exhaust (Let cool to 0 kg per sq. cm. before opening or venting).,4) Smooth magnetic stirring bar, teflon coated (1x5 cm),ExFLP-19,- 2 - April 1997,5) Stirrer hot plate,6) U.S. standard No. 230 sieve,7) Shallow pan > 20 cm in diameter,8) 2 L percolator, premarked at 250 and 1700 mL levels, or a 1 L Kilborn funnel,9) Wash bottles,10) Suction filtration apparatus with Buchner or Hirsch funnel (5-7 cm perforated plate),11) Ruled filter paper. Filter paper should be larger than funnel plate (7-9 cm),12) Petri dishes to suit size of filter paper used,13) Rubber policeman attached to a long glass rod,14) Stereoscopic microscope (10-30x),15) Ashless filter paper. Filter paper should be larger than funnel plate (7-9 cm),16) Drying oven (40-60oC),17) Crucible,18) Desiccator,19) Muffle oven,20) Concentrated HCl,21) Antifoam solution. Antifoam "B" - BDH Chemicals,22) Detergent, 1% sodium lauryl sulphate,23) Mineral oil. Paraffin oil, white, light 125/135 Saybolt Universal Viscosity (38oC), specific gravity,0.840-0.860 (24oC),24) Alcohol, either 95% ethanol or 99% isopropanol,25) Glycerol-alcohol (95%) mixture (1:1),26) (CHCl3) Chloroform,ExFLP-19,- 3 - April 1997,5. PROCEDURE,The examination shall be carried out in accordance with the following instructions.,5.1 Preparation of Analytical Units,5.1.1 In a random fashion from a sample unit weigh 50 g of white wheat flour. This 50 g constitutes an,analytical unit.,5.1.2 Repeat Step 5.1.1 for remaining two sample units.,5.2 Isolation - Light Filth,5.2.1 Transfer an analytical unit to a 2 L beaker.,5.2.2 Digest according to Step (a) or (b) below. If the flour is not adequately digested by (a) (as indicated,by lumps in the suspension) the flour should be digested instead by (b).,(a) Add 600 mL of HCl (18 mL concentrated HC1 + 582 mL water), and 0.3 mL of antifoam,solution and cover with a lid. Set beaker in a larger container in case of breakage.,Autoclave for 5 min at 121oC. Caution: check autoclave periodically for corrosive damage.,(b) Add 1 L of HCl (50 mL of concentrated HC1 and 950 mL water) and 0.3 mL of antifoam,solution. Digest by boiling mixture for 10 min on a stirrer hot plate with constant magnetic,stirring.,5.2.3 If the flour contains fat material floating on top of the HCl suspension or such that there is excessive,floating of tissue at the oil-water interface (Step 5.2.11 below), defat as follows. Wet sieve flour on a,No. 230 sieve with hot (50-70oC) tap water until rinse water is clear. Place sieve in a shallow pan,cover residue to a depth of approximately 2 cm with hot (50-70oC) detergent (1% sodium lauryl,sulphate), leave for 5 min, drain and wet sieve with hot tap water until all……
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